Lactate production measured by YSI and Western blot of CA-IX transfected U2-OS cells after 2hrs. Mitochondrial polarization using JC-1 mitochondrial dye in CA-IX clones ( N=8-12 per group, 2 bioreplicates Kruskal Wallis Test p<0.05*, p<0.01**, p<0.005***). ATP linked OCR measured by mitochondrial stress test XFe96 Seahorse assay, by injecting oligomycin to shut off mitochondrial respiration. YSI analysis of lactate in media at 24 and 48 hours in CA-IX clones ( N=6 replicates, Ordinary one-way ANOVA p<0.01**, 0.005***, 0.001****.). (Two-tailed Fisher’s exact t-test p<0.05*). Effect of PMA1 expression on experimental and spontaneous metastasis in MCF-7 cells. n=158-438 cells, standard deviation shown. Average intracellular pHi in control and CA-IX expressing MCF-7 cells as a function of extracellular pH. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Īdditional file 1: Table S1. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.

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4165 proton drive